Application
WB analysis of HeLa cell lysate using ATP-citrate lyase antibody [EP704Y] at a dilution of 1:5,000.| Product Name | ATP citrate lyase antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP704Y] to ATP-citrate lyaseATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ATP citrate lyase aa 1050-1150 (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP704Y |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | HumanMouseRatMarmoset |
| Concentration | 1mg/ml |
| Gene Synonyms | CLATP ATPCL ACL ACLY |
|---|---|
| Target | ATP-citrate lyase |
| Tissue Specificity | This antibody recognises ATP citrate lyase (ACL). |
Application
WB analysis of HeLa cell lysate using ATP-citrate lyase antibody [EP704Y] at a dilution of 1:5,000.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
