Application
Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ATP2B3 antibody at 1:500 dilution.| Product Name | ATP2B3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP2B3 (ATPase, Ca++ transporting, plasma membrane 3)The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ATP2B3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (99%), Rat (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PMCA3 antibody PMCA3a antibody ATP2B3 antibody plasma membrane calcium ATPase antibody plasma membrane calcium-transporting ATPase 3 antibody plasma membrane calcium pump antibody "ATPase Ca++ transporting plasma membrane 3 antibody" |
|---|---|
| Molecular Weight(MW) | 134 kDa (note) (3) |
| Cellular Localization | Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ATP2B3 antibody at 1:500 dilution.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
