Application
Immunofluorescence analysis of methanol-fixed HeLa, using ATP5J antibody at 1:500 dilution.Application
ATP5J antibody detects ATP5J protein at mitochondria in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ATP5J antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE diluted at 1:1000Application
ATP5J antibody detects ATP5J protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ATP5J antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using ATP5J antibody at 1:500 dilution.| Product Name | ATP5J antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP5J (ATP synthase-coupling factor 6, mitochondrial)Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Full length human ATP5J Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (91%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATP5 antibody ATP5A antibody ATPM antibody CF6 antibody F6 antibody ATP5J antibody "mitochondrial ATP synthase subunit F6 antibody" coupling factor 6 antibody "ATP synthase-coupling factor 6 mitochondrial antibody" "mitochondrial ATP synthase c |
|---|---|
| Molecular Weight(MW) | 13 kDa (note) (7) |
| Cellular Localization | Mitochondrion , Mitochondrion inner membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ATP5J antibody at 1:500 dilution.
Application
ATP5J antibody detects ATP5J protein at mitochondria in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ATP5J antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE diluted at 1:1000
Application
ATP5J antibody detects ATP5J protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ATP5J antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using ATP5J antibody at 1:500 dilution.| Positive Control | HeLa , HeLa membrane |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
