Application
Sample (30 ug of whole cell lysate) A: A431 B: Molt-4 7.5% SDS PAGE diluted at 1:1000Application
ATP6V0A4 antibody detects ATP6V0A4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V0A4 protein stained by ATP6V0A4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ATP6V0A4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V0A4 (ATPase, H+ transporting, lysosomal V0 subunit a4)This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V0A4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (89%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A4 antibody ATP6N1B antibody ATP6N2 antibody MGC130016 antibody MGC130017 antibody RDRTA2 antibody RTA1C antibody RTADR antibody STV1 antibody VPH1 antibody VPP2 antibody ATP6V0A4 antibody "vacuolar proton pump subunit 2 antibody" V-ATPase 11 |
|---|---|
| Molecular Weight(MW) | 96 kDa (note) (3) |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Molt-4 7.5% SDS PAGE diluted at 1:1000
Application
ATP6V0A4 antibody detects ATP6V0A4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V0A4 protein stained by ATP6V0A4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 , HeLa , Molt-4 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
