Application
Sample (30 µg of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP6V1E1 antibody at 1:250 dilution.Application
ATP6V1E1 antibody detects ATP6V1E1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V1E1 protein stained by ATP6V1E1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | ATP6V1E1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V1E1 (ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1)This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (98%), Xenopus laevis (82%), Chicken (87%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATP6E antibody ATP6E2 antibody ATP6V1E antibody P31 antibody Vma4 antibody ATP6V1E1 antibody "H(+)-transporting two-sector ATPase 31kDa subunit antibody" "H+-transporting ATP synthase chain E vacuolar antibody" vacuolar proton pump subunit E 1 an |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (3) |
Application
Sample (30 µg of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP6V1E1 antibody at 1:250 dilution.
Application
ATP6V1E1 antibody detects ATP6V1E1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V1E1 protein stained by ATP6V1E1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
