Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE diluted at 1:1000| Product Name | ATP6V1E1 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V1E1 (ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1)This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (98%), Chicken (85%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP6E antibody ATP6E2 antibody ATP6V1E antibody P31 antibody Vma4 antibody ATP6V1E1 antibody "H(+)-transporting two-sector ATPase 31kDa subunit antibody" "H+-transporting ATP synthase chain E vacuolar antibody" vacuolar proton pump subunit E 1 an |
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| Molecular Weight(MW) | 26 kDa (note) (3) |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HepG2 , Molt-4 , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
