Application
Western blot analysis of extracts from 293 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using ATRIP (Phospho-Ser224) Antibody. The lane on the left is treated with synthesized peptide.| Product Name | ATRIP (phospho Ser224) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATRIP (phospho Ser224)This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012] |
| Immunogen | Peptide sequence around phosphorylation site of serine 224 (H-V-S(p)-P-R) derived from Human ATRIP. |
| Modification | p-Ser224 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:3000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATRIP ATR interacting protein |
|---|---|
| Target | ATRIP (phospho Ser224) |
| Tissue Specificity | The antibody detects endogenous levels of ATRIP only when phosphorylated at serine 224. |
Application
Western blot analysis of extracts from 293 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using ATRIP (Phospho-Ser224) Antibody. The lane on the left is treated with synthesized peptide.| Application Notes | WB: 1:500 - 1:3000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
