Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000Application
AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Product Name | AUF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AUF1 (heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa))This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (98%), Rat (98%), Xenopus laevis (82%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AUF1 antibody AUF1A antibody HNRPD antibody P37 antibody hnRNPD0 antibody HNRNPD antibody "ARE-binding protein AUFI type A antibody" hnRNP D0 antibody heterogeneous nuclear ribonucleoprotein D0 antibody "heterogeneous nuclear ribonucleoprotein D |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000
Application
AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
