Application
WB analysis of extracts from various cell lines using Axin 2 antibody.Application
ICC/IF analysis of U2OS cells using Axin 2 antibody (red) and DAPI (blue).Application
IHC-P analysis of rat kidney using Axin 2 antibody at a dilution of 1:200.Application
IHC-P analysis of human lung cancer using Axin 2 antibody at a dilution of 1:200.| Product Name | Axin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Axin 2The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human AXIN2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ODCRCS axin 2 AXIL AXIN2 |
|---|---|
| Target | Axin 2 |
| Molecular Weight(MW) | 93 kDa (note) |
Application
WB analysis of extracts from various cell lines using Axin 2 antibody.
Application
ICC/IF analysis of U2OS cells using Axin 2 antibody (red) and DAPI (blue).
Application
IHC-P analysis of rat kidney using Axin 2 antibody at a dilution of 1:200.
Application
IHC-P analysis of human lung cancer using Axin 2 antibody at a dilution of 1:200.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
