Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:3000Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AXIN1 antibody [N1N2], N-term diluted at 1:1000.Application
AXIN1 antibody [N1N2], N-term detects AXIN1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AXIN1 antibody [N1N2], N-term diluted at 1:3000.| Product Name | AXIN1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AXIN1 (axin 1)This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human AXIN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (80%), Chicken (84%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AXIN antibody MGC52315 antibody AXIN1 antibody axin-1 antibody axis inhibitor 1 antibody axis inhibition protein 1 antibody "fused mouse homolog of antibody" "protein phosphatase 1 regulatory subunit 49 antibody" axin 1 antibody |
|---|---|
| Molecular Weight(MW) | 96 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:3000
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AXIN1 antibody [N1N2], N-term diluted at 1:1000.
Application
AXIN1 antibody [N1N2], N-term detects AXIN1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AXIN1 antibody [N1N2], N-term diluted at 1:3000.| Positive Control | A431 , PC-12 , Neuro 2A |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
