Application
AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody diluted at 1:500.Application
An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody at concentration ranged from 0.25 to 1000 ng/mL.Application
Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody diluted at 1:500.Application
AXL antibody detects AXL protein by western blot analysis. A. 30 μg A549 whole cell extract B. 30 μg H1299 whole cell extract C. 30 μg HCT116 whole cell extract 5 % SDS-PAGE AXL antibody dilution: 1:500Application
AXL antibody detects AXL protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human AXL-transfected 293T cells 7.5 % SDS-PAGE AXL antibody dilution: 1:5000Application
AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody dilution: 1:500.Application
AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. AXL antibody dilution: 1:500.Application
AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: AXL antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) . Blue: Nuclear staining with Hoechst 33342.| Product Name | AXL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AXL (AXL receptor tyrosine kinase)The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, PigMouse (87%), Rat (87%), Pig (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AXL receptor tyrosine kinase JTK11 UFO |
|---|---|
| Target | AXL |
| Molecular Weight(MW) | 98 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody diluted at 1:500.
Application
An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody at concentration ranged from 0.25 to 1000 ng/mL.
Application
Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody diluted at 1:500.
Application
AXL antibody detects AXL protein by western blot analysis. A. 30 μg A549 whole cell extract B. 30 μg H1299 whole cell extract C. 30 μg HCT116 whole cell extract 5 % SDS-PAGE AXL antibody dilution: 1:500
Application
AXL antibody detects AXL protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human AXL-transfected 293T cells 7.5 % SDS-PAGE AXL antibody dilution: 1:5000
Application
AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody dilution: 1:500.
Application
AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. AXL antibody dilution: 1:500.
Application
AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: AXL antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) . Blue: Nuclear staining with Hoechst 33342.| Positive Control | H1299 , human AXL-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
