Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using B3GALT4 antibody at 1:500 dilution.| Product Name | B3GALT4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to B3GALT4 (UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4)This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human B3GALT4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (81%), Rhesus Monkey (98%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BETA3GALT4 antibody GALT2 antibody GALT4 antibody B3GALT4 antibody b3Gal-T4 antibody gal-T2 antibody ganglioside galactosyltransferase antibody "beta-1 3-GalTase 4 antibody" "UDP-galactose:beta-N-acetyl-galactosamine-beta-1 3-galactosyltransferase |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane |
Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using B3GALT4 antibody at 1:500 dilution.| Positive Control | PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
