Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using B4GALT4 antibody at 1:250 dilution.| Product Name | B4GALT4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to B4GALT4 (UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4)This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human B4GALT4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, BovineMouse (88%), Rat (90%), Dog (92%), Rhesus Monkey (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B4Gal-T4 antibody beta4Gal-T4 antibody B4GALT4 antibody "beta-1 4-galactosyltransferase 4 antibody" "beta-1 4-GalTase 4 antibody" "UDP-galactose:beta-N-acetylglucosamine beta-1 4-galactosyltransferase 4 antibody" "UDP-Gal:beta-GlcNAc beta-1 4-galacto |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using B4GALT4 antibody at 1:250 dilution.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
