Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using BAD (Cleaved-Asp71) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BAD (Cleaved-Asp71) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | Bad (cleaved Asp71) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bad (cleaved Asp71)Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse BAD. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL2binding component6 BCL2-binding component 6 BCL2 ANTAGONIST OF CELL DEATHBCL2-antagonist of cell death protein BCL2binding protein BCL2-binding protein BCL2L8 BCL2 ANTAGONIST OF CELL DEATHBCL2antagonist of cell death protein BAD BCL2binding com |
|---|---|
| Target | Bad (cleaved Asp71) |
| Molecular Weight(MW) | 20 kDa (note) |
| Tissue Specificity | BAD (Cleaved-Asp71) Antibody detects endogenous levels of fragment of activated BAD resulting from cleavage adjacent to Asp71. |
| Cellular Localization | Upon phosphorylation, locates to the cytoplasm. |
Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using BAD (Cleaved-Asp71) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BAD (Cleaved-Asp71) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
