| Product Name | Bad (phospho Ser112) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bad (phospho Ser112)Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. |
| Modification | p-Ser112 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAD phospho Bad (pSer112) phospho BBC2 BAD (PhosphoSer112) phospho-Bad (pSer112) phosphoBBC6 phosphoBad (pSer112) phospho-Bad (pS112) BCL2L8 phospho Bad (pS112) BAD (Phospho-Ser112) phosphoBad (pS112) BAD (Phospho Ser112) Bad (phospho S112) B |
|---|---|
| Target | Bad (phospho Ser112) |
| Tissue Specificity | This antibody is specific for Bad (phospho Ser112). |
| Cellular Localization | Upon phosphorylation, locates to the cytoplasm. |
| Application Notes | Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
