Application
Western blot analysis of extracts from HT29 and HepG2 cells using BAD(Ab-136) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-136) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Product Name | Bad antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BadThe protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
| Immunogen | Peptide sequence around aa.134~138 (S-R-S-A-P) derived from Mouse BAD. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BBC2 BCL2L8 BAD bcl-2-binding component 6 bcl-2-like protein 8 BCL2-binding component 6 bcl2-L-8 BCL2-binding protein BCL2-antagonist of cell death protein bcl-XL/Bcl-2-associated death promoter bcl2 antagonist of cell death BCL-X/BCL-2 binding |
|---|---|
| Target | Bad |
| Molecular Weight(MW) | 23 (note) |
Application
Western blot analysis of extracts from HT29 and HepG2 cells using BAD(Ab-136) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-136) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
