| Product Name | Bad antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BadThe Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bad) apoptosis. Similar to other family member, Bad also exhibits sequence homology to Bcl-2. Bad can selectively dimerize with Bcl-xL and Bcl-2, but not with Bax, Bcl-xs, Mcl-1, or itself. In mammalian cells, Bad binds more strongly to Bcl-xL than Bcl-2. This may explain why Bad reverse the death repressor activity of Bcl-xL, but not that of Bcl-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIP |
| Western blot analysis (1-2 µg/ml), immunoprecipitation (4-8 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 22 kDa Bad protein and it does not cross-react with related family members.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bcl2like8 protein Bcl2binding component 6 Bcl2like 8 protein Bcl-2-like 8 protein Bcl2binding component6 BBC6 BCL2L8 Bcl-2-binding component 6 Bcl 2 like 8 protein Bcl 2 binding component 6 |
|---|---|
| Target | Bad |
| Tissue Specificity | This antibody is specific for Bad. |
| Application Notes | Western blot analysis (1-2 µg/ml), immunoprecipitation (4-8 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 22 kDa Bad protein and it does not cross-react with related family members.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
