Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE Bag1 antibody diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Bag1 antibody at 1:200 dilution.| Product Name | Bag1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bag1 (BCL2-associated athanogene)The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bag1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (82%), Rat (81%), Pig (87%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HAP antibody RAP46 antibody BAG1 antibody Bcl-2 associating athanogene-1 protein antibody Bcl-2-binding protein antibody BAG-1 antibody BAG family molecular chaperone regulator 1 antibody glucocortoid receptor-associated protein RAP46 antibody "rec |
|---|---|
| Target | Bag1 |
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Isoform 2: Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE Bag1 antibody diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Bag1 antibody at 1:200 dilution.| Positive Control | H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
