Application
Western blot analysis of extracts of MCF7 cell lines, using BAK1 antibody.Application
Immunofluorescence analysis of HeLa cell using Bak1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded rat heart using BAK1 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human stomach cancer using Bak1 antibody at dilution of 1:200 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human kidney cancer using Bak1 antibody at dilution of 1:200 (x400 lens).| Product Name | BAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAKThe protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human BAK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL2-antagonist/killer 1 CDN1 BAK1 BCL2L7 BAK-LIKE |
|---|---|
| Target | BAK |
| Molecular Weight(MW) | 23 kDa (note) |
Application
Western blot analysis of extracts of MCF7 cell lines, using BAK1 antibody.
Application
Immunofluorescence analysis of HeLa cell using Bak1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded rat heart using BAK1 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using Bak1 antibody at dilution of 1:200 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human kidney cancer using Bak1 antibody at dilution of 1:200 (x400 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
