Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DDX39 antibody [C1C3] detects DDX39 protein at cytoplasm and nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DDX39 antibody [C1C3] diluted at 1:500.Application
Immunoprecipitation of DDX39 protein from 293T whole cell extracts using 5 μg of DDX39 antibody [C1C3] . Western blot analysis was performed using DDX39 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | BAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BAT1This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX39. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rat, Xenopus laevis, Rhesus Monkey, BovineRat (98%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAT1 antibody BAT1L antibody DDX39 antibody DDXL antibody MGC18203 antibody MGC8417 antibody URH49 antibody DDX39A antibody "nuclear RNA helicase DECD variant of DEAD box family antibody" DEAD box protein 39 antibody "UAP56-related helicase 49 |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DDX39 antibody [C1C3] detects DDX39 protein at cytoplasm and nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DDX39 antibody [C1C3] diluted at 1:500.
Application
Immunoprecipitation of DDX39 protein from 293T whole cell extracts using 5 μg of DDX39 antibody [C1C3] . Western blot analysis was performed using DDX39 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
