Application
Immunofluorescence staining of methanol-fixed A549 cells using Bax (Phospho-Thr167) Antibody.| Product Name | Bax (phospho Thr167) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bax (phospho Thr167)The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of threonine167 (P-G-T(p)-P-T) derived from Human Bax. |
| Modification | p-Thr167 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| IF: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAX BCL2L4 BCL2-associated X protein |
|---|---|
| Target | Bax (phospho Thr167) |
| Tissue Specificity | The antibody detects endogenous levels of Bax only when phosphorylated at threonine 167. |
Application
Immunofluorescence staining of methanol-fixed A549 cells using Bax (Phospho-Thr167) Antibody.| Application Notes | IF: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
