Application
Western blot analysis of BBS4 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in A549 cell line lysate. BBS4 Antibody (Center) (arrow) was detected using the purified Pab.Application
Western blot analysis of BBS4 (arrow) using rabbit polyclonal BBS4 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BBS4 gene.| Product Name | BBS4 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BBS4 (Internal)This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by severe pigmentary retinopathy, obesity, polydactyly, renal malformation and mental retardation. The proteins encoded by BBS gene family members are structurally diverse. The similar phenotypes exhibited by mutations in BBS gene family members are likely due to the protein's shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene has sequence similarity to O-linked N-acetylglucosamine (O-GlcNAc) transferases in plants and archaebacteria and in human forms a multi-protein "BBSome" complex with six other BBS proteins. Alternative splice variants have been described but their predicted protein products have not been experimentally verified. |
| Immunogen | KLH conjugated synthetic peptide between 346~376 amino acids from the Center region of human BBS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Bardet-Biedl syndrome 4 protein BardetBiedl syndrome4 protein BBS4 BardetBiedl syndrome 4 protein |
|---|---|
| Target | BBS4 |
| Molecular Weight(MW) | 58.282 kDa (note) |
| Cellular Localization | Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm. |
Application
Western blot analysis of BBS4 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in A549 cell line lysate. BBS4 Antibody (Center) (arrow) was detected using the purified Pab.
Application
Western blot analysis of BBS4 (arrow) using rabbit polyclonal BBS4 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BBS4 gene.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
