Application
BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCA1 protein stained by BCA1 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. BCA1 antibody [N3C3] diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | BCA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCA1 (chemokine (C-X-C motif) ligand 13)B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANGIE antibody ANGIE2 antibody BCA-1 antibody BCA1 antibody BLC antibody BLR1L antibody SCYB13 antibody CXCL13 antibody B-cell chemoattractant antibody small-inducible cytokine B13 antibody b lymphocyte chemoattractant antibody "small inducible |
|---|---|
| Molecular Weight(MW) | 13 kDa (note) |
Application
BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCA1 protein stained by BCA1 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. BCA1 antibody [N3C3] diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
