Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 antibody at 1:100 dilution.Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 antibody at 1:500 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCAT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | BCAT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCAT2 (branched chain amino-acid transaminase 2, mitochondrial)This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCAT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (83%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCAM antibody BCATM antibody BCT2 antibody PP18 antibody BCAT2 antibody "branched chain aminotransferase 2 mitochondrial antibody" placental protein 18 antibody "branched-chain-amino-acid aminotransferase mitochondrial antibody" "branched chain a |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
| Cellular Localization | Isoform A: Mitochondrion , Isoform B: Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 antibody at 1:100 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2 antibody at 1:500 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCAT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , H1299 , HCT116 , MCF-7 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
