Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA antibody at 1:250 dilution.Application
BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCKDHA protein stained by BCKDHA antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining.| Product Name | BCKDHA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCKDHA (branched chain keto acid dehydrogenase E1, alpha polypeptide)The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Sheep, Chimpanzee, BovineMouse (95%), Rat (95%), Pig (94%), Sheep (95%), Chimpanzee (98%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCKDE1A antibody FLJ45695 antibody MSU antibody MSUD1 antibody OVD1A antibody BCKDHA antibody 2-oxoisovalerate dehydrogenase (lipoamide) antibody BCKDH E1-alpha antibody "2-oxoisovalerate dehydrogenase subunit alpha mitochondrial antibody" branch |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA antibody at 1:250 dilution.
Application
BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCKDHA protein stained by BCKDHA antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining.| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
