Application
WB analysis of human placenta tissue using BCKDHA antibody at 1μg/ml.Application
IHC-P analysis of human lung tissue using BCKDHA antibody at 1:100. Left to right:DAPI, BCKDHA, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.Application
WB analysis of MCF-7 cells using BCKDHA antibody at 0.2-1μg/ml.| Product Name | BCKDHA antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCKDHAThe branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human BCKDHA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSU OVD1A BCKDHA MSUD1 BCKDE1A |
|---|---|
| Target | BCKDHA |
| Molecular Weight(MW) | 50 KDa (note) |
Application
WB analysis of human placenta tissue using BCKDHA antibody at 1μg/ml.
Application
IHC-P analysis of human lung tissue using BCKDHA antibody at 1:100. Left to right:DAPI, BCKDHA, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
Application
WB analysis of MCF-7 cells using BCKDHA antibody at 0.2-1μg/ml.| Positive Control | human placenta , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
