Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:Raji 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK antibody at 1:100 dilution.| Product Name | BCKDK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCKDK (branched chain ketoacid dehydrogenase kinase)Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rhesus Monkey, BovineZebrafish (82%), Xenopus laevis (87%), Rhesus Monkey (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCKDK antibody branched-chain alpha-ketoacid dehydrogenase kinase antibody BCKD-kinase antibody branched chain alpha-ketoacid dehydrogenase kinase antibody "[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial antibody" BCKDHKIN a |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:Raji 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK antibody at 1:100 dilution.| Positive Control | HeLaS3 , Raji , mouse brain , rat kidney , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
