Application
Western blot analysis of BCL2 (pT69) expression in HepG2 UV-treated (A); HeLa (B); HL60 (C); THP1 (D); Jurkat paclitaxel-treated (E); NIH3T3 H2O2-treated (F); PC12 H2O2-treated (G) whole cell lysates.Application
Immunohistochemical analysis of BCL2 (pT69) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Bcl-2 (phospho Thr69) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bcl-2 (phospho Thr69)This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary. |
| Modification | p-Thr69 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL2 PPP1R50 B-cell CLL/lymphoma 2 Bcl-2 |
|---|---|
| Target | Bcl-2 |
| Tissue Specificity | Recognizes endogenous levels of BCL2 (pT69) protein. |
Application
Western blot analysis of BCL2 (pT69) expression in HepG2 UV-treated (A); HeLa (B); HL60 (C); THP1 (D); Jurkat paclitaxel-treated (E); NIH3T3 H2O2-treated (F); PC12 H2O2-treated (G) whole cell lysates.
Application
Immunohistochemical analysis of BCL2 (pT69) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
