Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2 antibody diluted at 1:1000.Application
BCL2 antibody detects BCL2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL2 protein stained by BCL2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Bcl-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bcl-2This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | CatCat (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B-cell CLL/lymphoma 2 PPP1R50 Bcl-2 |
|---|---|
| Target | BCL2 |
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane |
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2 antibody diluted at 1:1000.
Application
BCL2 antibody detects BCL2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL2 protein stained by BCL2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
