Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: BCL2 transfected 293T lysate 12% SDS PAGE diluted at 1:5000| Product Name | Bcl-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCL2 (apoptosis regulator Bcl-2)This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bcl-2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Bcl-2 antibody BCL2 antibody "protein phosphatase 1 regulatory subunit 50 antibody" apoptosis regulator Bcl-2 antibody B-cell CLL/lymphoma 2 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: BCL2 transfected 293T lysate 12% SDS PAGE diluted at 1:5000| Positive Control | Raji , BCL2 transfected 293T lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
