Application
Western blot analysis of Bcl-xL expression in Jurkat (Lane 1&2), mouse small intestine (Lane 3), and rat kidney (Lane 4) lysates.| Product Name | Bcl-xL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bcl-xLBcl-xL is a 30 kDa anti-apoptotic protein belonging to the Bcl-2 family. Bcl-xL prevents apoptosis through two different mechanisms: by heterodimerization with an apoptotic protein to inhibit its apoptotic effect, and by its direct pore-forming effect on the outer-membrane of mitochondria to help maintain a normal membrane state under stress conditions. Bcl-xL can be cleaved by caspases upon apoptotic activation, which converts Bcl-xL from an antiapoptotic protein to a proapoptotic cleavage product. The cleavage product of Bcl-xL forms cytochrome c-releasing pores in the mitochondrial membrane to facilitate the apoptosis signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (0.5-4 µg/ml) and immunoprecipitation (20 µ/ml). However, the optimal conditions should be determined individually. The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL2-like 1 protein BCL2L1 BclxL Bcl-xL BCL2like1 protein Bcl-xS BclxS BCL2like 1 protein Bcl xL Bcl xS BCL2 like 1 protein |
|---|---|
| Target | Bcl-xL |
| Tissue Specificity | This antibody is specific for Bcl-xL. |
Application
Western blot analysis of Bcl-xL expression in Jurkat (Lane 1&2), mouse small intestine (Lane 3), and rat kidney (Lane 4) lysates.| Application Notes | Western blotting (0.5-4 µg/ml) and immunoprecipitation (20 µ/ml). However, the optimal conditions should be determined individually. The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
