Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bcl-XS antibody diluted at 1:1000.Application
Bcl-XS antibody detects Bcl-XS protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Bcl-XS antibody dilution: 1:500.Application
Bcl-XS antibody detects Bcl-XS protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bcl-XS antibody dilution: 1:500.Application
Bcl-XS antibody detects Bcl-XS protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract D. 30 μg MCF-7 whole cell lysate/extract 12 % SDS-PAGE Bcl-XS antibody dilution: 1:1000| Product Name | Bcl-XS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bcl-XS (BCL2-like 1)The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bcl-XS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL-XL/S antibody BCL2L antibody BCLX antibody BCLXL antibody BCLXS antibody Bcl-X antibody DKFZp781P2092 antibody bcl-xL antibody bcl-xS antibody BCL2L1 antibody apoptosis regulator Bcl-X antibody bcl-2-like protein 1 antibody "protein phospha |
|---|---|
| Molecular Weight(MW) | 18.9KDa (note) |
| Tissue Specificity | Bcl-XS antibody was designed specific for BCL-XS |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane |
Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bcl-XS antibody diluted at 1:1000.
Application
Bcl-XS antibody detects Bcl-XS protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Bcl-XS antibody dilution: 1:500.
Application
Bcl-XS antibody detects Bcl-XS protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bcl-XS antibody dilution: 1:500.
Application
Bcl-XS antibody detects Bcl-XS protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract D. 30 μg MCF-7 whole cell lysate/extract 12 % SDS-PAGE Bcl-XS antibody dilution: 1:1000| Positive Control | A549 , H1299 , HCT116 , MCF-7 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
