Application
Western blot analysis of extracts from COS7 tissue using Bcr (Phospho-Tyr360) antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Bcr (phospho Tyr360) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bcr (phospho Tyr360)A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 360 (T-T-Y(p)-R-M) derived from Human Bcr. |
| Modification | p-Tyr360 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCR D22S11 PHL ALL breakpoint cluster region D22S662 BCR1 CML |
|---|---|
| Target | Bcr (phospho Tyr360) |
| Tissue Specificity | The antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 360. |
Application
Western blot analysis of extracts from COS7 tissue using Bcr (Phospho-Tyr360) antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
