Application
ApplicationApplication
Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 at a dilution of 1:1000 overnight at 4°C on a rocking platform.Application
Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 , at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.| Product Name | beta 1 Adrenergic Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta 1 Adrenergic ReceptorAdrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines. |
| Immunogen | Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| ICC/IF: 1:100: |
|
| Species Reactivity | HumanMouseRatCatPrimate |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Synonyms | beta1 Adrenergic receptor Adrb-1 betaAR beta 1 Adrenergic receptor 11554 beta-AR Adrb1 Adrb 1 beta AR |
|---|---|
| Target | beta 1 Adrenergic Receptor |
Application
Application
Application
Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 at a dilution of 1:1000 overnight at 4°C on a rocking platform.
Application
Immunofluorescent analysis of ADR-Beta-1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1 , at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.| Application Notes | ICC/IF: 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | crude serum with 0.05% sodium azide. |
