Application
beta A3 Crystallin antibody detects beta A3 Crystallin protein by western blot analysis. A. 50 μg mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000| Product Name | beta A3 Crystallin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Beta crystallin A3 (crystallin, beta A1)Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq] |
| Immunogen | Full length human beta A3 Crystallin Recombinant protein. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Rabbit, Chicken, Rhesus Monkey, Bovine, Guinea pigHuman (100%), Rat (95%), Dog (96%), Rabbit (96%), Chicken (84%), Rhesus Monkey (97%), Bovine (93%), Guinea pig (95%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRYB1 antibody CRYBA1 antibody beta-crystallin A3 antibody "crystallin beta A3 antibody" eye lens structural protein antibody "crystallin beta A1 antibody" |
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| Molecular Weight(MW) | 25 kDa (note) |
Application
beta A3 Crystallin antibody detects beta A3 Crystallin protein by western blot analysis. A. 50 μg mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000| Positive Control | 293T , A431 , H1299 , HeLa , Molt-4 , Raji , mouse fetal eye |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
