Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:1000.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:5000.Application
beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. beta Actin antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin antibody at 1:500 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Japanese medaka, Dog, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigRat (100%), Japanese medaka (100%), Dog (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 antibody ACTB antibody "actin cytoplasmic 1 antibody" beta cytoskeletal actin antibody PS1TP5-binding protein 1 antibody "actin beta antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:1000.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:5000.
Application
beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. beta Actin antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin antibody at 1:500 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Positive Control | 293T , A431 , HeLa , HepG2 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
