Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:10000Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. Beta Actin antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE diluted at 1:10000Application
Immunoprecipitation of beta Actin protein from A431 whole cell extracts using 5 μg of beta Actin antibody . Western blot analysis was performed using beta Actin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:15000.Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: PC-12 10% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta Actin antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using beta Actin antibody at 1:500 dilution.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Japanese medaka, Xenopus laevis, Dog, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigJapanese medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 antibody ACTB antibody "actin cytoplasmic 1 antibody" beta cytoskeletal actin antibody PS1TP5-binding protein 1 antibody "actin beta antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:10000
Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. Beta Actin antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE diluted at 1:10000
Application
Immunoprecipitation of beta Actin protein from A431 whole cell extracts using 5 μg of beta Actin antibody . Western blot analysis was performed using beta Actin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:15000.
Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: PC-12 10% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta Actin antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using beta Actin antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
