Application
beta Actin antibody detects beta Actin protein at cytoplasm in rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. beta Actin antibody diluted at 1:500.Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:1000.Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC:1:50~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Japanese medaka, Xenopus laevis, Dog, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigJapanese medaka (100%), Xenopus laevis (100%), Dog (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | actin beta ACTB BRWS1 PS1TP5BP1 |
|---|---|
| Target | beta Actin |
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
beta Actin antibody detects beta Actin protein at cytoplasm in rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. beta Actin antibody diluted at 1:500.
Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:1000.
Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC:1:50~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
