Application
Beta Actin antibody detects beta Actin protein at cytoplasm in mouse thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. Beta Actin antibody diluted at 1:500.Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Beta Actin antibody diluted at 1:500.Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. Beta Actin antibody diluted at 1:500.Application
Beta Actin antibody detects beta Actin protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Beta Actin antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: THP-1 10% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:10000Application
beta Actin antibody detects beta Actin protein at cytosol on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. beta Actin antibody dilution: 1:500.Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:10000.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Japanese medaka, Xenopus laevis, Dog, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigRat (100%), Japanese medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 antibody ACTB antibody "actin cytoplasmic 1 antibody" beta cytoskeletal actin antibody PS1TP5-binding protein 1 antibody "actin beta antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Beta Actin antibody detects beta Actin protein at cytoplasm in mouse thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. Beta Actin antibody diluted at 1:500.
Application
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Beta Actin antibody diluted at 1:500.
Application
Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. Beta Actin antibody diluted at 1:500.
Application
Beta Actin antibody detects beta Actin protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Beta Actin antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: THP-1 10% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:10000
Application
beta Actin antibody detects beta Actin protein at cytosol on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. beta Actin antibody dilution: 1:500.
Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:10000.| Positive Control | Jurka , Raji , THP-1 , NIH-3T3 , 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
