Application
Western blot analysis of extracts from HT29 cells using b-Catenin(Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using beta -Catenin (Phospho-Ser37) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 cells using beta -Catenin (Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide .| Product Name | beta Catenin (phospho Ser37) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Catenin (Phospho Ser37)Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway. |
| Immunogen | Peptide sequence around phosphorylation site of serine 37 (I-H-S(p)-G-A) derived from Human beta -Catenin. |
| Modification | p-Ser37 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CTNNB DKFZp686D02253 FLJ25606 FLJ37923 CTNNB1 catenin beta-1 catenin (cadherin-associated protein) beta 1 88kDa |
|---|---|
| Target | beta Catenin (Phospho Ser37) |
| Molecular Weight(MW) | 92 (note) |
Application
Western blot analysis of extracts from HT29 cells using b-Catenin(Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using beta -Catenin (Phospho-Ser37) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells using beta -Catenin (Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
