Western blot analysis of extracts from SW626 cells using b-Catenin(Phospho-Thr41/Ser45) (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Rabbit Polyclonal antibody to beta Catenin (Phospho Thr41/Ser45)Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
Immunogen
Peptide sequence around phosphorylation site of threonine 41/serine 45 (A-T-T(p)-T-A-P-S(p)-L-S) derived from Human beta -Catenin.
Modification
p-Thr41/Ser45
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Western blot analysis of extracts from SW626 cells using b-Catenin(Phospho-Thr41/Ser45) (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.