Application
Madin Darby Bovine kidney (MDBK) cells were separated on SDS-PAGE and probed with AB to Catenin Beta (RB) (Cat. No. ). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody dilution 1/8,000| Product Name | beta Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to beta CateninThe distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues (1,2,3) bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. (6,7) They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. (4,5) Absence of alpha-catenin is found in certain tumor cell lines (8) and reduced levels in certain human carcinomas (9). Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. (10) This interaction involves a 15-aa repeat in the APC. (11) Beta-catenin cell levels seem to be controlled by APC. (12) The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. (13) |
| Immunogen | Synthetic peptide: PGDSNQLAWFDTDL conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse β Catenin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-FWB |
| Dot: Use at an assay dependant dilution. IHC-Fr: Use at a dilution of 1/2000 (bovine kidney frozen sections or cultured MDBK cells). WB: Use at a dilution of 1/4000 (MDBK cells extract). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatZebra FishFishXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTNNB1 P35222 114550 1499 beta Catenin CTNNB |
|---|---|
| Target | beta Catenin |
| Tissue Specificity | Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA.In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide.Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901). |
| Cellular Localization | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
Application
Madin Darby Bovine kidney (MDBK) cells were separated on SDS-PAGE and probed with AB to Catenin Beta (RB) (Cat. No. ). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody dilution 1/8,000| Positive Control | MDBK cells |
|---|---|
| Application Notes | Dot: Use at an assay dependant dilution. IHC-Fr: Use at a dilution of 1/2000 (bovine kidney frozen sections or cultured MDBK cells). WB: Use at a dilution of 1/4000 (MDBK cells extract). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Whole Serum |
