Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using beta Tubulin 2 antibody at 1:200 dilution.Application
beta Tubulin 2 antibody [N1C1] detects beta Tubulin 2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. beta Tubulin 2 antibody [N1C1] dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:5000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:3000| Product Name | beta Tubulin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin 2 (tubulin, beta 2A)Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TUBB antibody, TUBB2 antibody, dJ40E16.7 antibody, TUBB2A antibody, "tubulin, beta polypeptide 2 antibody", tubulin beta-2A chain antibody, class IIa beta-tubulin antibody, "tubulin, beta 2A antibody" |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using beta Tubulin 2 antibody at 1:200 dilution.
Application
beta Tubulin 2 antibody [N1C1] detects beta Tubulin 2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. beta Tubulin 2 antibody [N1C1] dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:5000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:3000| Positive Control | 293T , A431 , Jurkat , Raji , NIH-3T3 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
