Application
Sample(30 ug whole cell lysate) A:A431 B:Hep G2 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000Application
beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin protein stained by beta Tubulin antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | beta Tubulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta 1)Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Cat, Pig, Chicken, BovineCat (81%), Pig (88%), Chicken (86%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TUBB1 antibody, "beta tubulin 1, class VI antibody", tubulin beta-1 chain antibody, class VI beta-tubulin antibody, "tubulin, beta 1 antibody" |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
Sample(30 ug whole cell lysate) A:A431 B:Hep G2 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000
Application
beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin protein stained by beta Tubulin antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
