Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BLM antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
BLM antibody [C3], C-term detects BLM protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLM protein stained by BLM antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | BLM antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BLM (Bloom syndrome, RecQ helicase-like)The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human BLM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BS antibody, MGC126616 antibody, MGC131618 antibody, MGC131620 antibody, RECQ2 antibody, RECQL2 antibody, RECQL3 antibody, BLM antibody, "DNA helicase, RecQ-like type 2 antibody", Bloom syndrome protein antibody, recQ protein-like 3 antibody, "Bloom syndro |
|---|---|
| Molecular Weight(MW) | 159 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BLM antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
BLM antibody [C3], C-term detects BLM protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLM protein stained by BLM antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
