Application
Sample (30 ug of whole cell lysate) A: 293T B : A431 5% SDS PAGE diluted at 1:1000Application
BLM antibody [N1N3] detects BLM protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLM protein stained by BLM antibody [N1N3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Product Name | BLM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BLM (Bloom syndrome, RecQ helicase-like)The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BLM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BS antibody, MGC126616 antibody, MGC131618 antibody, MGC131620 antibody, RECQ2 antibody, RECQL2 antibody, RECQL3 antibody, BLM antibody, "DNA helicase, RecQ-like type 2 antibody", Bloom syndrome protein antibody, recQ protein-like 3 antibody, "Bloom syndro |
|---|---|
| Molecular Weight(MW) | 159 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: 293T B : A431 5% SDS PAGE diluted at 1:1000
Application
BLM antibody [N1N3] detects BLM protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLM protein stained by BLM antibody [N1N3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
