Application
Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2μg/ml.Application
Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody at 1μg/ml.| Product Name | BNIP3L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BNIP3LMembers in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designatedBnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA ofBnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. |
| Immunogen | peptide (DAQHESGQSSSRGSSH)corresponding to amino acids 77 to 92 of human origin,which are identical to those of mouse Bnip3L (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Western blot at 0.5 to 1 μg/ml.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Gene Synonyms | 605368 665 O60238 BNIP3L BNIP3a NIX |
|---|---|
| Target | BNIP3L |
Application
Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2μg/ml.
Application
Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody at 1μg/ml.| Positive Control | K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4). |
|---|---|
| Application Notes | Western blot at 0.5 to 1 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
