Rabbit Polyclonal antibody to Bone SialoproteinThe protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human Bone Sialoprotein
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Dog: 79%; Human: 100%
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Synonyms
BNSP SP II BSP IBSP BSP II
Target
Bone Sialoprotein
Molecular Weight(MW)
35 KDa (note)
Application
Application
WB analysis of HepG2 cells using Bone Sialoprotein antibody at 1.25μg/ml.
Positive Control
HepG2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.