Application
Western Blot: BP1 Antibody [ - Detection of MDA-MB-231 cell lysates.Application
Immunohistochemistry: BP1 Antibody [ - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast cancer cells.Application
Immunoprecipitation: BP1 Antibody [ - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.| Product Name | BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BP1DLX (Distal-less) genes are essential for early development. BP1 (beta protein 1) is a homeodomain-containing isoform of DLX4. Strong BP1 expression is seen in placenta and kidney tissue, with no expression found in any other tissue (1). This protein has been shown to be activated in 80% of invasive ductal breast tumors and is associated with breast cancer progression ( 2, 3). It is also expressed in the bone marrows of 63% of acute myeloid leukemia (AML) patients (4). In addition, BP1 plays a role in globin gene regulation by repressing the beta- globin gene (1). |
| Immunogen | A synthetic peptide made specifically to the human BP1 protein sequence (between residues 1-60).There is no homology with the peptide sequences used for immunogen of this antibody and the DLX4 or DLX7 proteins. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| This antibody has been tested in immunohistochemistry and immunoprecipitation of 35S-labeled BP1 protein only. It is not known if this antibody will immunoprecipitate a cell extract. Other applications have not been tested. Immunohistochemistry: 1:50-1:100*Optimal working dilutions should be determined by the investigator.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | BP1 DLX9 DLX8 601911 Q92988 DLX4 DLX7 1748 |
|---|---|
| Target | BP1 |
| Tissue Specificity | This antibody is specific for human BP1. |
Application
Western Blot: BP1 Antibody [ - Detection of MDA-MB-231 cell lysates.
Application
Immunohistochemistry: BP1 Antibody [ - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast cancer cells.
Application
Immunoprecipitation: BP1 Antibody [ - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.| Application Notes | This antibody has been tested in immunohistochemistry and immunoprecipitation of 35S-labeled BP1 protein only. It is not known if this antibody will immunoprecipitate a cell extract. Other applications have not been tested. Immunohistochemistry: 1:50-1:100*Optimal working dilutions should be determined by the investigator.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. Avoid freezing. |
| Storage Buffer | This antibody is supplied as 0.1 ml of peptide affinity purified antibody containing 0.1% sodium azide.**Blocking peptide is not available. |
