Application
Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM antibody at 1:100 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse spleen 12% SDS PAGE diluted at 1:1000| Product Name | BPGM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BPGM (2,3-bisphosphoglycerate mutase)2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BPGM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rabbit, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (92%), Rabbit (97%), Chicken (81%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BPGM antibody "erythrocyte 2 3-bisphosphoglycerate mutase antibody" "2 3-diphosphoglycerate mutase antibody" "2 3-bisphosphoglycerate synthase antibody" bisphosphoglycerate mutase antibody BPG-dependent PGAM antibody "2 3-bisphosphoglycerate mutase |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (3) |
Application
Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM antibody at 1:100 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse spleen 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
